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Banhos,Aureo; Hrbek,Tomas; Gravena,Waleska; Sanaiotti,Tânia; Farias,Izeni P.. |
We report the characterization and optimization of 45 heterologous microsatellite loci, and the development of a new set of molecular sex markers for the conservation and management of the Neotropical harpy eagle (Harpia harpyja L. 1758). Of the 45 microsatellites tested, 24 were polymorphic, six monomorphic, 10 uncharacterizable due to multiple bands and five did not amplify. The observed gene diversity of the analyzed sample of H. harpyja was low and similar to that of other threatened Falconiformes. While a high proportion of the microsatellite markers were highly variable, individuals of H. harpyja could be differentiated by a joint analysis of just three (p = 2.79 x 10-4) or four markers (p = 2.89 x 10-5). Paternity could be rejected with 95.23% and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation genetics; Harpia harpyja; Microsatellites; Raptors; Sex markers. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100025 |
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L.,María Doris Escobar; Farias,Izeni P.; B.,Donald C. Taphorn; Landines,Miguel; Hrbek,Tomas. |
The arowanas, fishes of Gondwanan origin, are represented in South America by the genus Osteoglossum. All species were initially reported as being exclusive to the Amazon region, with O. ferreirai restricted to the Negro River basin and O. bicirrhosum to the Amazon and Essequibo Rivers basin. Starting in the mid 1970's it was reported that O. ferreirai also occurs in the Orinoco River basin. In all regions the arowanas assumed significant socio-economic importance due to their popularity in the international ornamental fish trade, leading to over-exploitation of both species in some areas. The Orinoco populations are particularly heavily exploited, and thus conservation and management measures are needed. Both depend on the clarification of taxonomic... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aruana; Biological introduction; Black arowana; Silver arowana. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000200335 |
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Frederico,Renata G.; Farias,Izeni P.; Araújo,Maria Lúcia Góes de; Charvet-Almeida,Patricia; Alves-Gomes,José A.. |
The family Potamotrygonidae is monophyletic comprising three genera: Paratrygon Duméril, Potamotrygon Garman and Plesiotrygon Rosa, Castello & Thorson. The distribution of most species in this family is restricted to a single basin or fluvial system. Only Potamotrygon motoro, Potamotrygon orbignyi and Paratrygon aiereba are found in more than one river basin. In this study we investigate genetic structuring of Paratrygon aiereba, from five rivers of the Amazon region: Negro, Solimões-Amazon-Estuary system, Tapajós, Xingu and Araguaia. Sixty-three individuals were sequenced for ATPase 6, and a representative subsample of 27 individuals was sequenced for COI. The COI dataset analysis indicated that Paratrygon is sister to all other potamotrygonid... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amazon basin; ATPase; COI; Population genetics. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000100007 |
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Vasconcelos,William R.; Hrbek,Tomas; Silveira,Ronis da; Thoisy,Benoit de; Marioni,Boris; Farias,Izeni P.. |
The genetic structure of Caiman crocodilus was investigated using a 1085 bp mtDNA fragment of the cytochrome b gene. Inferences were based on 125 individuals from nine localities in Peru, Brazil and French Guiana. With the exception of Mamirauá Lake, Anavilhanas Archipelago and the Tapará Community which show a signal of demographic expansion, the sampled localities are in a mutation-drift genetic equilibrium. Divergence between the Amazon basin and extra-Amazon basin localities is significant; however, inference from Nested Clade Analysis cannot distinguish between continuous range expansion, long distance colonization or past fragmentation; however, past fragmentation is unlikely due to low number of mutational steps separating these two regions. The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic structure; Phylogeography; Genetic diversity; Demographic expansion; Cytochrome b; Caiman crocodilus. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200006 |
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